Abstract: γ-Alumina supportedβ-Mo2N0.78 catalysts were synthesized via temperature-program-med nitriding reaction of MoO3 using N2-H2 mixture gas. The precursor of active phases was prepared by impregnation of γ-Alumina with aqueous solution of ammonium heptamolybdate((NH4)6Mo7O24·4H2O), followed by drying and calcination in air. The hydrodesulfurization(HDS) reaction of thiophene over these catalysts was performed in a fixed-bed reactor at atmosphere pressure. The influence of HDS reaction temperature, pre-reduction of H2 and the addition of Co and Ni on the activity of β-Mo2N0.78/γ-Al2O3 were studied. The results show that the HDS activity increases with increasing reaction temperature from 320 ℃ to 400 ℃. Pre-reduction can deactivate the activity of catalyst. The addition of Co and Ni can improve the catalytic activity ofβ-Mo2N0.78/γ-Al2O3 catalyst.