Abstract: The experiment of hydrogen production from catalytic steam reforming of bio-oil aqueous fraction was carried out in a bench scale fixed-bed reactor. The effects of temperature and sorbents were investigated. The optimal reaction temperatures for catalytic steam reforming of bio-oil aqueous fraction with and without sorbents were determined. The results show that at the best reaction conditions for bio-oil aqueous fraction reforming is at 800℃ under atmosphere pressure, at which, up to 60% of H₂ and 10% of carbon monoxide in the effluent gas were obtained. Absorption of carbon dioxide increased hydrogen content in the effluent gas as well as hydrogen yield. 85% of hydrogen content  was reached using CaO as sorbent at optimized reaction temperature of 600℃.Due to the decrease of the capacity for carbon dioxide capture by CaO at 650℃ during the reaction, the enhancement to  hydrogen yield dropped significantly.