Abstract: Co-based catalyst was prepared by impregnation method with SiO₂ as the support. The effects of glucose and Pd on the structure and property of the catalysts were characterized by N₂ low-temperature adsorption, XRD, SEM and H₂-TPR. The reactive performance of the catalysts in F-T synthesis was evaluated in a fixed-bed reactor. The shape of Co₃O₄ crystal particle was changed by the added glucose and Pd. The addition of glucose increased the dispersion of Co₃O₄ species, and the addition of Pd promoted the reduction of the catalyst although its dose was only 0.0125%. The combination of glucose and Pd increased the dispersion and reduction of Co₃O₄ species on the catalysts simultaneously. As the result, both of the CO conversion and the selectivity of C₅₊ hydrocarbons increased in F-T synthesis reaction.